Immunogen: A synthesized peptide derived from human Ku70. Uniprot: >>Visit The Human Protein Atlas. Gene ID: Gene Name: XRCC6. Molecular Weight: Observed Mol.Wt.: 70kD.

Anti-Ku70/XRCC6 Antibody PA1642. Uniprot Id: P12956: NCBI Gene Id: 2547: Species Of This Entry: Human: Gene Name: XRCC6

ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity). KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.

a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). Knockdown of Ku70 was achieved by transfecting HeLa cells with Ku70 specific siRNAs (Silencer® select Product # s5457, s5455). Western blot analysis (Fig. a) was performed using nuclear enriched cell extracts from the Ku70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). HSF1 interacted directly with both of the N-terminal sequences of the Ku70 and Ku86 proteins, which inhibited the endogenous heterodimeric interaction between Ku70 and Ku86.

Knockdown of Ku70 was achieved by transfecting HeLa with Ku70 specific siRNAs (Silencer® select Product # S5455, S5457). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1).

Ku70 binding activity was localized to the C-terminal coiled-coil domain of nCLU. Leucine residues 357, 358, and 361 of nCLU were necessary for Ku70-nCLU interaction. The N- and C-terminal coiled-coil domains of nCLU interacted with each other, suggesting that the protein could dimerize or fold.

Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement.

Identification of Ku70 and Ku80 homologues in Arabidopsis thaliana: evidence for a role in the repair of DNA double-strand breaks. Tamura K. , Adachi Y. , Chiba K. , Oguchi K. , Takahashi H. In higher organisms such as mammals and plants, DNA double-strand breaks (DSBs) are repaired preferentially by non-homologous end joining (NHEJ) rather than by homologous recombination.

PLA0263 Sigma-Aldrich Rabbit anti-Ku70 Antibody, Affinity Purified Powered by Bethyl Laboratories, Inc. Synonym: 5′-dRP lyase Ku70, 5′-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, 70 kDa subunit, 70kDa, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase II, CTC box binding factor 75 kDa subunit, CTC box Mar 5, 2021 KU70; TLAA; ML8; ATP-Dependent DNA Helicase II, 70 KDa Subunit by UniProt/SwissProt and Phenotype-based relationships between UniProt accession no. P12956.

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU70, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA (By similarity).
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Target Names, XRCC6. Alternative Names, 5''-deoxyribose- 5-phosphate lyase Ku70 antibody; 5''-dRP lyase Ku70 antibody; 70 kDa subunit annotation (IEA) methods that are supplied by the UniProt-GOA project to the GO Consortium: InterPro, IPR006164, Ku70/Ku80 beta-barrel domain. InterPro Ku70 has been referred to by several names including: Lupus Ku autoantigen protein p70; ATP-dependent DNA helicase 2 subunit 1; X-ray repair complementing X-ray repair cross-complementing protein 6 (KU70). Mol. type, Protein.

However, Rad3/Rad26 must have additional target(s), as cells lacking Tel1/Rad32, Rad1/Rad9/Hus1/Rad17, and Ku70 groups did not circularize chromosomes. Ku70 binding activity was localized to the C-terminal coiled-coil domain of nCLU. Leucine residues 357, 358, and 361 of nCLU were necessary for Ku70-nCLU interaction. The N- and C-terminal coiled-coil domains of nCLU interacted with each other, suggesting that the protein could dimerize or fold.
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Ku70; Ku70, bridge and pillars domain superfamily; Ku70/Ku80, N-terminal alpha/beta; Ku70/Ku80 beta-barrel domain; Ku70/Ku80 C-terminal arm; UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information. Q23976; UniProt

X-ray repair cross complementing 6 [Source:HGNC Symbol;Acc: HGNC:4055]. Gene Synonyms. D22S671, D22S731, G22P1, KU70, ML8. Location. Recombinant Human Ku70 protein is a Wheat germ Full length protein 1 to 609 aa range and validated in WB, ELISA, SDS-PAGE. The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the UNIPROT ALIGN P12956. N P E G K V Single-stranded DNA-dependent ATP-dependent helicase.

Anti-Ku70/XRCC6 Antibody PA1642. Uniprot Id: P12956: NCBI Gene Id: 2547: Species Of This Entry: Human: Gene Name: XRCC6

Human Ku70/80 associates physically with telomerase through interaction with hTERT. Chai W., Ford L.P., Lenertz L., Wright W.E., Shay J.W. Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8).

Required also for telomere recombination to repair telomeric ends in the absence of telomerase.